Repopulation of autophagy-deficient stromal cells with autophagy-intact cells after repeated breeding in uterine mesenchyme-specific Atg7 knockout mice

Objective: Autophagy is highly active in ovariectomized mice experiencing hormone deprivation, especially the uterine mesenchyme. responsible for turnover of vasoactive factors uterus, which was demonstrated anti-Müllerian receptor type 2 (<i>Amhr2</i>)-Cre-driven autophagy-related gene 7 (<i>Atg7</i>) knockout (<i>Amhr-Cre/Atg7<sup>f/f</sup></i> mice). In that study, we uncovered a striking difference amount sequestosome 1 (SQSTM1) accumulation between virgin and breeder with same genotype. Herein, aimed to determine whether repeated breeding changed composition mesenchymal cell populations stroma.Methods: All female used this study were <i>Atg7</i> deleted by <i>Amhr2</i> promoter-driven Cre recombinase stroma myometrium, except triangular stromal region on mesometrial side. <i>Amhr-Cre/Atg7<sup>f/f</sup></i> divided into two groups: no mating history aged 11 12 months, at least 6-month cycles multiple pregnancies around months. The uteri Western blotting immunofluorescence staining. Results: SQSTM1 accumulation, representing deletion halted autophagy, much higher than breeders. Breeders showed reduced several vasoconstrictive factors, are potential autophagy targets, suggesting repopulated autophagy-intact cells during pregnancies. Conclusion: Multiple seem have improved environment replacing autophagy-deficient cells, providing evidence mixing.